000			01936nam a2200241Ia 4500
003			ASM
005			20241206092903.0
008			241203s9999 xx 000 0 und d
010			_a2006041110
020			_a9781588290724
020			_qHardback
040			_cASM
050			_a
100			_a" Katja M. Arndt," _eAuthor
245		0	_aProtein Engineering Protocols _c" Katja M. Arndt, Kristian M. Müller."
260			_a"Totowa, N.J." _b"Humana Press," _c2007
300			_a312 Pages _billustrations _c24 cm
520			_a"Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins. Divided into two sections, Protein Engineering Protocols reviews rational protein design strategies and directed evolutionary techniques, and their impact on protein engineering. The first section presents design and computational strategies for protein engineering focusing on designing a protein with desired properties, including examples covering a wide range of engineering techniques, such as protein-protein interactions, DNA binding, antibody mimics, and enzymatic activity. The second section on evolutionary techniques provides step-by-step instructions on library design and statistical assessment of library quality. New methods for DNA shuffling as well as different selection strategies are also presented. Following the successful Methods in Molecular Biology series format, this volume provides a comprehensive guide to the methods used at every stage of the engineering process. By combining a thorough theoretical foundation with detailed protocols, Protein Engineering Protocols will be invaluable to all research workers in the area, from graduate students to senior investigators."
546			_aEnglish
650			_aProtein engineering.
700			_a"Müller, Kristian M." _eEditors
942			_cBooks
999			_c4325 _d4325